Author:
RANJAN R,PRIYADHARSINI R,GOEL A K,SINGH BREJENDRA,KUMAR SATISH,KHARCHE S D,JINDAL S K
Abstract
Chlorpromazine hydrochloride (CPH) acts as a membrane stabilizer during semen freezing. The ejaculates from Jamunapari bucks (2–4 years old) maintained at this institute under semi intensive management system were utilized to find out the freezability of buck semen at different levels of CPH by conventional method of freezing. The ejaculates were collected twice a week using artificial vagina and were extended to maintain sperm concentration approximately 100 million per dose (0.25 ml) with Tris- Citric acid- Fructose (TCF) diluent. The post thaw motility, live sperm count, abnormalities, acrosomal integrity and hypo-osmotic swelling positive spermatozoa did not differ significantly at different levels of CPH. There was no significant effect of CPH inclusion in goat semen dilutor on post thaw qualities. None of the levels of CPH tested improved sperm survival, and the highest level of drug (400 µM) was found to be spermicidal.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference9 articles.
1. Bhattacharyya H K, Ganai N A and Khan H M. 2012. Fertility of local goats of Kashmir using frozen semen of Boer bucks. Wudpecker Journal of Agricultural Research 1(8): 346–48.
2. Foote R H and Gray L C. 1960. Effect of promazine hydrochloride and chlorpromazine hydrochloride on the motility and fertility of bovine semen. Journal of Dairy Science 43: 1499–504.
3. Hong C Y, Chaput de Saintonge D M and Turner P. 1982. Effects of chlorpromazine and other drugs acting on the central nervous system on human sperm motility. European Journal of Clinical Pharmacology 22(5): 413–16.
4. Kharche S D, Jindal S K, Priyadharsini R, Satish Kumar, Goel A K, Ramachandran N and Rout P K. 2013. Fertility following frozen semen artificial insemination in Jamunapari goats. Indian Journal of Animal Sciences 83(10): 1071–73.
5. Paudel K P, Kumar S, Meur S K and Kumaresan A. 2010. Ascorbic acid, catalase and chlorpromazine reduce cryopreservation- induced damages to crossbred bull spermatozoa. Reproduction in Domestic Animal 45(2): 256–62.