Author:
SINGH RANDHIR,KAUR SIMRANPREET,TOMAR J S,GILL J P S
Abstract
Antibiotic resistance is a cause of concern worldwide. Community settings are important reservoir of drug resistant microorganisms like Staphylococcus aureus. The present study was to determine the prevalence, phenotypic and genotypic antibiotic resistance pattern of S. aureus isolated from different community settings of university campus. A total of 300 swab samples were collected for isolation of S. aureus from different community settings at university campus of Guru Angad Dev Veterinary and Animal Sciences University and Punjab Agriculture University, Ludhiana, India. Confirmed S. aureus isolates were further subjected to antibiotic sensitivity by Epsilometer test (E-test) and detection of antibiotic resistance genes. The prevalence of S. aureus in the community samples was 12% (36/300). Methicillin Resistant S. aureus (MRSA) contamination among community was 3.33% (10/300). Among S. aureus isolates from community samples 63.8% (23/36) and all the MRSA isolates were multidrug resistant (MDR). Five out of 10 MRSA carried SCCmec type IVa, and 4 were pvl positive gene, therefore, designated as community associated MRSA (CA-MRSA). Phenotypic resistance to antibiotics ciprofloxacin, chloramphenicol, ceftriaxone clindamycin and trimethoprim-sulfamethoxazole was 69.4% (MIC ≥32 μg/ml), 63.9% (MIC 32 μg/ ml), 16.7% (MIC 16–64 μg/ml), 16.7% (MIC 256 μg/ml) and 8.3% (MIC 12–64 μg/ml), respectively. Resistance genes blaZ, mecA, tetK, tetM, ermB and aacA-aphD were present. Presence of MRSA and MDR variant in community settings is a public health concern, as cell phone, offices telephone, computer keyboard and tap faucet are commonly shared or touched by people. Therefore, have potential to disseminate widely, not only in the community settings but also in hospitals environment, complicating treatment.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference21 articles.
1. Al-Abdalall A H. 2010. Isolation and identification of microbes associated with mobile phones in Dammam in eastern Saudi Arabia. Journal of Family and Community Medicine 17(1): 11–14.
2. Aso J O, Kolawole D O, Aso O O and Ajisebutu S O. 2011. Antibiotic sensitivity pattern of Staphylococcus aureus from fomites in the Obafemi Awolowo University Teaching Hospital Complex (OAUTHC) Nigeria. International Journal of Medical Science 3(2): 32–36.
3. Dan Dan Sun, Xiao Xue Ma, Jian Hu, Yuan Tian, Long Pang, Hong Shang and Long Zhu Cui. 2013. Epidemiological and molecular characterization of community and hospital acquired Staphylococcus aureus strains prevailing in Shenyang, Northeastern China. Brazilian Journal of Infectious Diseases 17(6): 682–90.
4. Duran N, Ozer B, Duran G G, Onlen Y and Demir C. 2010. Antibiotic resistance genes and susceptibility patterns in staphylococci. Indian Journal of Medical Research 135: 389– 96.
5. Frye J G and Fedorka-Cray P. 2007. Prevalence, distribution and characterisation of ceftiofur resistance in Salmonella enterica isolated from animals in the USA from 1999 to 2003.