Author:
PATEL S J,JOSHI D V,RAVAL S H,PATEL B J,PATEL J G,CHAUHAN H C,CHANDEL B S,PATEL B K,SHAH N M
Abstract
The present study was carried out to study pathology and virulence of Pasteurella multocida B:2 isolated from natural outbreak of Pasteurella infection in rabbits. Healthy Pasteurella multocida B:2 free rabbits (12) were divided in control and challenged group. The challenged group rabbits were inoculated intra-nasally by spraying of 0.5 ml of inoculum in each nares, containing 1 × 105 CFU of Pasteurella multocida B:2 in BHI. The control group of rabbits was inoculated with 0.5 ml uncultured BHI broth in each nares in the same manner. Live animals were observed for atleast 14 days. The main clinical signs observed in infected rabbits comprised of conjunctivitis, nasal discharge, pyrexia, sneezing, dyspnoea and abdominal breathing. The pathomorphological changes in lungs comprised of acute fibrinous and/or fibrino suppurative bronchopneumonia/pleuropneumonia. Trachea showed acute inflammatory changes with multifocal erosion/ulcer and necrosis. Nasal cavity showed acute inflammation with accumulation of cellular debris in nasal meatus. Three infected rabbits showed meningitis characterized by congestion, infiltration of heterophils and minimal oedema. Pasteurella multocida B:2 was successfully detected in blood and tissues of infected rabbits by PCR. The organisms were also demonstrated in blood/impression smear from different organs.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference24 articles.
1. Al -Haddawi M H, Jasni S, Israf D A and Mutalib A R. 2001. Ultrastructural pathology of nasal and tracheal mucosa of rabbits experimentally infected with Pasteurella multocida serotype D:1. Research in Veterinary Science 70 (3): 191–97.
2. Dagleish M P, Finlayson J, Bayne C, MacDonald S, Sales J and Hodgson J C. 2010. Characterization and time course of pulmonary lesions in calves after intratracheal infection with Pasteurella multocida A:3. Journal of Comparative Pathology 142 (2-3): 157–69.
3. DiGiacomo R F, Deeb B J, Bernard B L, Klaassen J M and Chengappa M M. 1987. Safety and efficacy of a streptomycin dependent live Pasteurella multocida vaccine in rabbits. Laboratory Animal Science 37 (2): 187–90.
4. Dillehay D L, Paul K S, Digiacomo R F and Chengappa M M. 1991. Pathogenicity of Pasteurella multocida A:3 in Flemish Giant and New Zealand White rabbits. Laboratory Animals 25 (4): 337–41.
5. Dowling A, Hodgson J C, Schock A, Donachie W, Eckersall P D and Mckendrick I J. 2002. Experimental induction of pneumonic pasteurellosis in calves by intratracheal infection with Pasteurella multocida biotype A:3. Research in Veterinary Science 73 (1): 37–44.