Author:
REZA A M M T,LEE S J,SHIWANI S,SINGH N K
Abstract
Cytoplasmic lipid droplets (CLD's) formation is critical for lactation and health and so it could be detrimental in upholding the formation of CLD's in bovine mammary epithelial cells (MAC-T). We therefore, treated MAC-T cells with differentiation medium containing alpha-linolenic acid (ALA) (100 μM) and thiazolidenediones (TZD's)(10 μM) and observed CLD's formation in cellular cytoplasm with Oil-red-O staining and elution index percentage. We also observed significant up-regulation of adipogenic and down regulation of epithelial markers. In conclusion, 100 μM ALA plus 10 μM TZD's resulted in formation of CLD's and subsequent differentiation of MAC-T cells.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology