Cytotoxic potential of rhizome extracts of Hedychium spicatum L. in HepG2 cell line using MTT-Reference-Cited by-同舟云学术

Cytotoxic potential of rhizome extracts of Hedychium spicatum L. in HepG2 cell line using MTT

Published:2017-03-21 Issue:3 Volume:87 Page:
ISSN:2394-3327
Container-title:The Indian Journal of Animal Sciences
language:
Short-container-title:Indian J of Anim Sci

Author:

CHOUDHARY GOVIND KUMAR,SINGH SATYA PAL

Abstract

The present study was designed to evaluate the cytotoxic effects of extract of Hedychium spicatum rhizome extract on human liver hepatocellular carcinoma (HepG2) cell line using MTT assay. The crude extract of H. spicatum was prepared by cold maceration method, filtered, concentrated in different organic solution and tested for phytochemical evaluation and finally on HepG2 cell line. Dose-dependent cytotoxic activities were exhibited on HepG2 cell line. As the dose of the extract increased, the number of viable cells decreased. This confirms the cytotoxic potential of the rhizome of H. spicatum. The IC50 value of different extracts was determined and concluded that the methanolic extract had better potential than the other extracts.

Publisher

Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture

Subject

General Veterinary,Animal Science and Zoology

Reference8 articles.

1. Alison M R. 2005. Liver stem cells: implications for hepatocarcinogenesis. Stem Cell Reviews 1(3): 253–60.

2. Busuttil R W and Farmer D G. 1996. The surgical treatment of primary hepatobiliary malignancy. Liver Transpl Surgery 2(5 Suppl 1):114–30.

3. Edeoga H O, Okwu D E and Mbaebie B O. 2005. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4: 685–88.

4. Fatope M O, Ibrahim H and Takeda Y. 1993. Screening of higher plants reputed as pesticides using the brine shrimp lethality assay. International Journal of Pharmacognosy 31: 250 –54.

5. Penn I. 1991. Hepatic transplantation for primary and metastatic cancers of the liver. Surgery 110(4): 726–34.

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India;Journal of Ethnopharmacology;2023-12

2. In vitro hepatoprotective efficacy of extract of Hedychium spicatum rhizome in paracetamol induced toxicity in HepG2 cell line;The Indian Journal of Animal Sciences;2018-05-23