Effect of buffalo PDC-109 on caudal epididymal spermatozoa of bubaline species-Reference-Cited by-同舟云学术

Effect of buffalo PDC-109 on caudal epididymal spermatozoa of bubaline species

Published:2021-07-28 Issue:12 Volume:90 Page:1584-1588
ISSN:0367-8318
Container-title:The Indian Journal of Animal Sciences
language:
Short-container-title:Indian J of Anim Sci

Author:

RAMTEKE S S,RAJORIYA J S,SHENDE A M,GHOSH S K,PRASAD J K,PERUMAL P,SINGH M

Abstract

A study was conducted to assess the effect of buffalo seminal PDC-109 on its cauda epididymal spermatozoa. PDC-109 protein was purified by applying two-step chromatography procedures and included into epididymal spermatozoa. Epididymal ejaculates were splited into four groups as Gr 1: Control (without PDC), Gr 2: 20 Î¼g/mL PDC-109, Gr 3: 40 Î¼g/mL PDC-109 and Gr 4: 80 Î¼g/mL PDC-109. Semen quality parameters (SQPs) and in vitro fertility assay were evaluated. Significant improvement in post-thaw SQPs was observed in Gr 2 than in Gr 3, however, Gr 1 had significantly higher value than other groups. It is concluded that PDC-109 has dose dependent effect as increased dose causes detrimental effect in buffalo cauda epididymal sperm.

Publisher

Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture

Subject

General Veterinary,Animal Science and Zoology

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