Author:
RAMESHA K P,BASAVARAJU M,GEETHA G R,RAO AKHILA,HATKAR D N,KATAKTALWARE M A,SURESH K P,ANANTHARAJ A,DAS D N,DIVYA P
Abstract
A study was undertaken to analyze associations between HSP70 genetic variants of promoter region with heat tolerance and performance in Deoni (Bos indicus) cattle using PCR-SSCP technique. The analysis revealed three SSCP patterns, viz. AA, AB and BB with frequencies of 0.712, 0.254 and 0.034, respectively. Sequence analysis of representative samples of each pattern showed 7 SNPs, which included four transitions (A859G, A1046G, C1257T and G1294A) and 3 transversions (A897C, A1127C and G1166T). Cows with different SSCP patterns showed similar production and reproduction performances. The studies on physiological parameters and calculation of Heat tolerance coefficient (HTC) of individual cows indicated that Deoni cows maintained at Bengaluru were not under heat stress even after 6 h of exposure to sun during summer (March to May).
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference14 articles.
1. Banks A, Looper M L, Reiter S, Starkey L, Flores R, Hallford D and Rosenkrans C Jr. 2007. Identification of single nucleotide polymorphisms within the promoter region of the bovine heat shock protein 70 gene and associations with pregnancy. American Society Animal Science Southern Section Meeting 85: 10.
2. Barani A, Ramesha K P, Basavaraju M, Akhila Rao and Narasimha Rao T V L. 2015. Genetic characterization and identification of single nucleotide polymorphisms of ATP1A1 gene in Indian humped cattle. Indian Journal of Animal Research 10.8805/ ijar.7082
3. Beatty D T, Barnes A, Taylor E, Pethick D, McCarthy M and Maloney S K. 2006. Physiological responses of Bos taurus and Bos indicus cattle to prolonged, continuous heat and humidity. Journal of Animal Science 84: 972–85.
4. Bernabucci U, Lacetera N, Baumgard L H, Rhoads R P, Ronchi B and Nardone A. 2010. Metabolic and hormonal acclimation to heat stress in domesticated ruminants. Animal 7: 1167–83.
5. Board P J, Roberts J and Evans. 2009. The genetic control of erythrocyte reduced glutathione in Australian merino sheep. Journal of Agriculture Science 82: 395–98.