Author:
SINGH AMIT,SHANKER DAYA,JAISWAL AMIT KUMAR,KUMAR SANJEEV
Abstract
Antibody detection enzyme immunoasssay using whole cyst lysate antigen of Sarcocystis fusiformis was standardized and evaluated for the diagnosis of bubaline sarcocystosis. For the study, tissue (oesophagus, tongue, heart and diaphragm) and blood samples from each of the 160 buffaloes slaughtered at local abattoir of Mathura, Uttar Pradesh, India, were collected. Tissue samples were subjected to macroscopic and microscopic examinations for the detection of sarcocysts whereas blood samples were used for sera extraction for detection of antibodies by ELISA. Sarcocystis fusiformis isolated from tissues were used for preparation of whole cyst lysate antigen and subsequently used for development of antibody-detection ELISA. Ab-ELISA was standardized using pooled known negative, pooled known positive, hyper immune and foetal calf serum and was then compared with tissue examination. Antibody-ELISA could detect antibodies against Sarcocystis in 63.75% serum samples with 88.88% sensitivity and 68.57% specificity. Kappa value showed moderate efficacy of the WCL based antibody ELISA.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference11 articles.
1. Dubey J P, Calero-Bernal R, Rosenthal B M, Speer C A and Fayer
2. R. 2015. Sarcocystosis of Animals and Humans, second ed. CRC Press, Inc., Boca Raton, Florida. Pp. 481.
3. Fayer R. 2004. Sarcocystis spp. in human infections. Clinical Microbiological Reviews 17: 894–902.
4. Hettiarachchi D C and Rajapakse R P V J. 2008. Antigenic analysis of bovine Sarcocystis spp. in Sri Lanka. Journal of the National Science Foundation of Sri Lanka 36: 239–44.
5. Kalita M, Sarmah P C and Singh S. 2015. Evaluation of modified agglutination test in detection of antibodies against Sarcocystis cruzi and Sarcocystis hirsuta of Cattle. International Journal of Recent Scientific Research 6: 5968–70.