Cryopreservation and freezability of epididymal and ejaculated stallion spermatozoa

Author:

TALLURI TR,JHAMB DINESH,PAUL NILENDU,MEHTA S C,SINGH JITENDAR,DEDAR R K,LEGHA R A,PAL YASH

Abstract

The aim of the present study was to compare the seminal attributes and freezability of the epididymal sperm recovered from castrated stallions and ejaculated semen collected through artificial vagina from the same stallions previously. The seminal quality parameters viz., viability, sperm morphology, plasma membrane functional integrity, acrosome integrity and mitochondrial membrane potential were studied, and recorded immediately after fresh semen collection/retrieval, before freezing, and after cryopreservation. Similar protocols were followed for freezing the epididymal and ejaculated semen. From the current study, it was observed that the sperm concentration of the epididymal recovered was significantly higher than that of fresh ejaculation. Other seminal parameters in thefresh sample, like total and progressive sperm motility, sperm membrane functional integrity, acrosome integrity and kinematic parameters like VSL, STR, and linearity were similar without any significant differences between both. However, mitochondrial membrane potential, BCF and linearity of the spermatozoa differed significantly between the groups after cryopreservation. From the current study, it can be concluded that the protocols used for cryopreserving the fresh ejaculates can be adopted for freezing the stallion epididymal spermatozoa without causing significant damage and through this method; elite stallion germplasm can be safely cryopreserved and conserved.

Publisher

Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture

Subject

General Veterinary,Animal Science and Zoology

Reference31 articles.

1. Alvarenga M A, Papa F O, Landim-Alvarenga F and Medeiros A. 2005. Amides as cryoprotectants for freezing stallion semen: A review. Animal Reproduction Science 89(1-4): 105–13.

2. Alvarez J G and Storey B T. 1982. Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility. Biology of Reproduction 27(5): 1102–08.

3. Amann R, Thompson Jr D, Squires E and Pickett B. 1979. Effects of age and frequency of ejaculation on sperm production and extragonadal sperm reserves in stallions. Journal of Reproduction and Fertility. Supplement(27): 1–6.

4. Bruemmer J, Moore A and Squires E. 2004. Freezing epididymal sperm from transported testicles. Transporting Gametes and Embryos 61.

5. Bruemmer J, Reger H, Zibinski G and Squires E. 2002. Effect of storage at 5°C on the motility and cryopreservation of stallion epididymal spermatozoa. Theriogenology 58(2-4): 405–07.

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