Author:
YADAV PARUL,KHARCHE S D,GOEL A K,JINDAL S K
Abstract
The present study was designed to compare the developmental competence of in vitro fertilized caprine embryos at different cell stages in three different culture medium(TCM-199, mSOF, KSOM). Oocytes were collected from ovaries and matured in TCM-199 medium containing FSH (5 μg/ml), LH (5 μg/ml) and estradiol-17β (1 μg/ml), supplemented with 20% fetal bovine serum at 38.5 °C and 5% CO2 in an incubator under humidified air for 27 h. After 27 h of in vitro maturation (IVM), oocytes were denuded, washed and co-incubated with 50 μl neat semen diluted in 5 ml of sperm TALP containing 20% EGS and 10μg/ml heparin. Oocytes-sperm after 18h of co-incubation were randomly divided into three groups: Group1(TCM-199 containing 10% NCS and 4mg/ml bovine serum albumin), Group 2(Synthetic oviductal fluid containing 10% NCS and 4mg/ml BSA) and Group 3(Potassium simplex optimization medium (KSOM) containing 10% NCS and 4mg/ml BSA) and found the cleavage rate, morulae and blastocyst percentage for groups 1, 2, and 3 were 13.6, 10.14 and 2.41%, 11.7, 7.6 and 3.84% and 31.4, 12.73 and 6.05%, respectively. Statistical analysis revealed that the cleavage rate and embryo development up to morula and blastocyst stage in group 3(KSOM) was significantly higher (P<0.01%) as compare to group1 and group2.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
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