Analysis of biomarkers for prediction of idiopathic infertility in Large White Yorkshire boar (Sus scrofa domesticus)

Abstract

The experiment was conducted to analyze biomarkers like pH, calcium (Ca2+), cholesterol (Ch), estradiol (E2) and vitamin D (Vit-D) in the serum for prediction of idiopathic infertility and its early amelioration. pH of epididymal luminal fluids (ELF) and concentrations of Ca2+, Ch, E2 and Vit-D were measured in serum, as well as in epididymal luminal fluids (ELF) and sperm cytosolic fluids (SCF) from caput, corpus and cauda epididymis of healthy, mature Large Yorkshire breed of boar (n=100). The levels of these bio-molecular components in the serum, in ELF and SCF from different epididymal segments were used statistically to develop a Curve Fit Regression Equations and a prediction equation to predict the quantum of these bio-molecules in the ELF and SCF by estimating their levels in the serum which would have been very difficult otherwise to be estimated in live animals. Further, from the clinical point of view, present findings about the spermatozoa maturation can pave the way for development of specific marker(s) that will assist in the development of new arena for both the prediction and early diagnosis of male infertility and for improving treatment modalities of male infertility, since epididymal dysfunctions are related to cases of idiopathic male infertility.