Differential karyotype profiling of three popular breeds of dogs in India

Abstract

The present investigation aims to study the karyology of the three most popular dog breeds as well as indigenous local dog. In this study, we identified the most popular dog breeds of the Punjab region which are maintained as companion animals, or for guarding. Metaphase plates were prepared after culturing of lymphocytes isolated from heparinized blood collected from the identified three most popular canine breeds. The isolated lymphocyte cells were cultured for 70-72 h following the cell cycle arrest at metaphase. The G-banding of the chromosomes was done by Giemsa staining through a standard protocol. The most popular three breeds of dog in the sub-tropical region were Labrador, the German Shepherd, and Pug. There were no significant distinguishable differences between the karyotypes of the dog breeds studied. This study gives insight into karyology information, which can be beneficial to the researchers, dog breeders, and kennel clubs. Moreover, it provides information about chromosomal abnormalities which may lead to the study of various fertility, growth, and phenotypic abnormalities problems in dog breeds.