Investigation of Spermatozoa and Seminal Plasma by Fourier Transform Infrared Spectroscopy

Author:

Barčot O.1,Balarin M.1,Gamulin O.1,Ježek D.1,Romac P.1,Brnjas-Kraljević J.1

Affiliation:

1. University of Zagreb, Medical School, Šalata 3, Zagreb, 10000, Croatia (O.B.); University of Zagreb, Medical School, Dept. of Physics and Biophysics, Šalata 3, Zagreb, 10000, Croatia (M.B., O.G., J.B.-K.); University of Zagreb, Medical School, Dept. of Histology and Embryology, Šalata 3, Zagreb, 10000, Croatia (D.J.); and Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, University of Zagreb, Medical School, Dugi dol 4a, Zagreb, 10000, Croatia (P.R.)

Abstract

Fourier transform infrared (FT-IR) spectra of human spermatozoa and seminal plasma were recorded and analyzed. The procedure that was established for sample preparation enabled acquisition of reproducible spectra. The parameter I1087/ I966 for controlling spectra reproducibility was defined. The assignment of bands was carried out using an empirical approach and the origin of the “sperm specific doublet”, the bands at 968 cm−1 and 981 cm−1, was determined. The principal component regression (PCR) algorithm was used to define the specific spectral regions correlating to characteristics of spermatozoa, such as concentration, straight-line velocity (VSL), and beat cross frequency (BCF). Then, simple spectral parameters, such as band intensities and band ratios, were tested to determine which one best correlates to characteristics of spermatozoa. The region of the amide I band, between 1700 cm−1 and 1590 cm−1, was defined as a specific spectral region that correlates to the concentration of spermatozoa. The parameter that gave the linear dependence to the concentration of spermatozoa was the intensity of the amide I band. For VSL, the bands between 1119 cm−1 and 943 cm−1 were defined as the specific spectral region. The relative amount of nucleic acids with respect to proteins showed linear dependence on the straight-line velocity of spermatozoa. BCF showed the best correlation to the bands between 3678 cm−1 and 2749 cm−1, which largely represent lipids and proteins. These results suggest that FT-IR spectroscopy can serve as an adjunct to conventional histopathology studies.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

Cited by 22 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3