Design and Applications of Rapid-Scan Spectrally Resolved Fluorescence Microscopy-Reference-Cited by-同舟云学术

Design and Applications of Rapid-Scan Spectrally Resolved Fluorescence Microscopy

Published:2000-12 Issue:12 Volume:54 Page:1727-1733
ISSN:0003-7028
Container-title:Applied Spectroscopy
language:en
Short-container-title:Appl Spectrosc

Author:

Haralampus-Grynaviski Nicole M.¹,Stimson Michael J.¹,Simon John D.¹

Affiliation:

1. Department of Chemistry, Duke University, Durham, North Carolina 27708 (N.M.H.-G., M.J.S., J.D.S.); and Department of Biochemistry, Duke University Medical Center, Duke University, Durham, North Carolina 27710 (J.D.S.)

Abstract

This paper addresses the design and applications of a spectrally resolved confocal fluorescence microscope that measures spectral information for each pixel in the image with a fast multi-point approach. The combined spatial and spectral resolution of the device is demonstrated by performing two-photon imaging of mixtures of fluorescently labeled microspheres. Overlapping emission spectra of dye solutions contained in microcapillaries are readily distinguished. Images of a human skin tissue section and a mouse kidney section are presented which demonstrate that the structure and spectra of biologic samples can be resolved.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

Link

http://journals.sagepub.com/doi/pdf/10.1366/0003702001948989

Reference27 articles.

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3. Measurement of intracellular pH and pCa with a confocal microscope

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