Gas-Phase Detection of Trinitrotoluene Utilizing a Solid-Phase Antibody Immobilized on a Gold Film by Means of Surface Plasmon Resonance Spectroscopy-Reference-Cited by-同舟云学术

Gas-Phase Detection of Trinitrotoluene Utilizing a Solid-Phase Antibody Immobilized on a Gold Film by Means of Surface Plasmon Resonance Spectroscopy

Published:2003-08 Issue:8 Volume:57 Page:906-914
ISSN:0003-7028
Container-title:Applied Spectroscopy
language:en
Short-container-title:Appl Spectrosc

Author:

Bowen J.¹,Noe L. J.¹,Sullivan B. P.¹,Morris K.¹,Martin V.¹,Donnelly G.¹

Affiliation:

1. Department of Chemistry, University of Central Oklahoma, Edmund, Oklahoma 73034 (J.B.); Department of Chemistry, University of Wyoming, Laramie, Wyoming 82070 (L.J.N., B.P.S., K.M., V.M.); Critical Angle, LLC, P.O. Box 472, Laramie, Wyoming 82073 (J.B., L.J.N., B.P.S.); and Laramie Police Department, Bomb Squad, 620 Plaza Ct., Laramie, Wyoming 82070 (G.D.)

Abstract

A multilayered biosensor was constructed and found to detect trinitrotoluene (TNT) in ppb concentrations in air both prior to and after detonation of TNT without use of a liquid phosphate buffered saline (PBS) superstrate. The biosensor surface was fabricated from a monoclonal antibody for TNT covalently bound to an 11,11′-dithio-bis(succinimidoylundecanoate) (DSU) self-assembled monolayer immobilized on a thin gold film bonded to a BK7 glass slide. The binding between the immobilized antibody and TNT antigen was detected using surface plasmon resonance spectroscopy (SPRS). Biosensor specificity for TNT was demonstrated with chemical homologues as well as against an unrelated explosive, RDX.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

Link

http://journals.sagepub.com/doi/pdf/10.1366/000370203322258850

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