Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria-Reference-Cited by-同舟云学术

Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria

Published:2004-01 Issue:1 Volume:58 Page:33-40
ISSN:0003-7028
Container-title:Applied Spectroscopy
language:en
Short-container-title:Appl Spectrosc

Author:

Zeiri L.¹,Bronk B. V.¹,Shabtai Y.¹,Eichler J.¹,Efrima S.¹

Affiliation:

1. Department of Chemistry, Ben-Gurion University of the Negev, P. O. Box 653, Beer-Sheva, Israel 84105 (L.Z., S.E.); AFRL/HEST, Building E5951, Aberdeen Proving Ground, Maryland 21010-5424 (B.V.B.); The Ilse Katz Center for Meso and Nanoscale Science and Technology (S.E.); Bioengineering Center, Tufts University, Medford, Massachusetts 02155 (Y.S.); and Department of Life Sciences, Ben-Gurion University of the Negev, P. O. Box 653, Beer-Sheva, Israel 84105 (J.E.)

Abstract

Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

Link

http://journals.sagepub.com/doi/pdf/10.1366/000370204322729441

Reference56 articles.

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