Spectroradiometer—Luminometer for Chemiluminescence and Fluorescence Quantum-Yield Studies

Abstract

A spectroradiometer–luminometer has been constructed, calibrated, and applied to the measurement of fluorescence- and chemiluminescence-emission intensity as a function of wavelength and to kinetic decay studies. It is also applicable to investigations of phosphorescence, electroluminescence, bioluminescence, and the spectroradiometry of external light sources. A computer program has been set up to take care of wavelength-response corrections and to convert emission intensities into photochemical, radiometric, or luminous units, including total integrated intensities for quantum-yield studies.