Tools for Combinatorial Chemistry: In situ Infrared Analysis of Solid-Phase Organic Reactions

Abstract

Methodology for in situ infrared monitoring and analysis of solid-phase organic reactions has been developed. The real-time, single-bead capabilities of this technique are illustrated through the analysis of solvent diffusion into, and subsequent wash-out from, a 70-μm aminomethyl polystyrene bead. This report further illustrates application of this technology for solid-phase synthesis with real-time analysis using two example reactions. Analytical hardware and the inherent benefits of real-time analysis with respect to precursor referencing and the quality, utility, and interpretation of the infrared data are also discussed.