Rapid Detection of Bacterial Growth by Infrared Spectrophotometry-Reference-Cited by-同舟云学术

Rapid Detection of Bacterial Growth by Infrared Spectrophotometry

Published:1991-09 Issue:8 Volume:45 Page:1366-1367
ISSN:0003-7028
Container-title:Applied Spectroscopy
language:en
Short-container-title:Appl Spectrosc

Author:

Iqbal Mohammad S.¹,Rashid Farooq¹

Affiliation:

1. Directorate of Nuclear Fuel and Materials, Pakistan Atomic Energy Commission, P.O. Box 1114, Islamabad, Pakistan (M.S.I.); and Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, P.O. Nilore, Islamabad, Pakistan (F.R.)

Abstract

A rapid and sensitive method based on measurement of carbon dioxide released from a culture medium as a result of bacterial metabolism of glucose is reported. The rate of release of carbon dioxide was measured by recording the absorbance at 2353 cm−1 (4.25 μm) against time using a standard infrared spectrophotometer. Detection of growth from only five organisms of Salmonella typhosa was significant after just fifteen minutes. In about three hours a clear pattern of growth was obtained by this method. The results are reproducible with a standard deviation around ±0.5%.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

Link

http://journals.sagepub.com/doi/pdf/10.1366/0003702914335850

Reference8 articles.

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3. Early Detection of Bacterial Growth, with Carbon-14-Labeled Glucose

4. THE DETECTION OF BACTERIA AND VIRUSES IN LIQUIDS

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