Raman Imaging Using a Tunable Dual-Stage Liquid Crystal Fabry-Perot Interferometer

Abstract

A 11 12 cm−1 bandpass electronically tunable liquid crystal dual-stage Fabry-Perot filter (DFPF) has been developed for use in Raman and fluorescence microscopic imaging. The imaging performance of the filter was demonstrated in the fluorescence and Raman modes. In the fluorescence mode, the filter was shown to give sharpened images using the nearest-neighbors deblurring algorithm. In the Raman imaging mode, background subtraction was shown to be useful for removal of the fluorescence from Raman images. With the use of a 100 × /1.25 objective, no significant decrease in the spatial resolution of the microscope was observed when the DFPF was inserted in the optical system.