Fluorescence Pattern Analysis Based on the Time-Resolved Ratio Method-Reference-Cited by-同舟云学术

Fluorescence Pattern Analysis Based on the Time-Resolved Ratio Method

Published:1991-05 Issue:4 Volume:45 Page:560-565
ISSN:0003-7028
Container-title:Applied Spectroscopy
language:en
Short-container-title:Appl Spectrosc

Author:

Wang Xue Feng¹,Uchida Teruo¹,Maeshima Muneo¹,Minami Shigeo¹

Affiliation:

1. Department of Applied Physics, Faculty of Engineering, Osaka University, Suita, Osaka 565, Japan

Abstract

A new technique is described for component pattern analysis based on time-resolved fluorescence image detection and a ratio method calculation. Differences in fluorescence lifetimes of components are positively utilized to estimate the spatial distribution of each component. Simulation results of the ratio method for fluorescence pattern analysis are discussed. Spatial deconvolution of multicomponent fluorescence data is rapidly carried out from a set of time-resolved, two-dimensional fluorescence data. An example of the application of a multigate time-resolved ratio method for fluorescence pattern analysis is provided. From the results, it is shown that the new method can be successfully used for multicomponent fluorescence pattern analysis at a reasonable signal-to-noise ratio.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

Link

http://journals.sagepub.com/doi/pdf/10.1366/0003702914336804

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