On-Line Detection in Slab Gel Electrophoresis Using Thermal Lens Spectrophotometry

Abstract

Thermal lens spectrophotometry was used to detect hemoglobin and cytochrome c within an acrylamide gel matrix both during electrophoresis and in static experiments in which each protein was incorporated in the matrix during polymerization. For cytochrome c, the detection limit was 0.02 mg/mL for static experiments (approximately 0.5 ng in the laser beam volume), while a detection limit of 180 ng (5 ng in the beam volume) was found for electrophoresis runs.