Flow-Injection Chemiluminescence Method for the Determination of Hydrocortisone and Progesterone-Reference-Cited by-同舟云学术

Flow-Injection Chemiluminescence Method for the Determination of Hydrocortisone and Progesterone

Published:1997-10 Issue:10 Volume:51 Page:1484-1487
ISSN:0003-7028
Container-title:Applied Spectroscopy
language:en
Short-container-title:Appl Spectrosc

Author:

Li Huai-Na¹,Ci Yun-Xing¹

Affiliation:

1. Department of Chemistry, Peking University, Beijing 100871, P.R. China (Y.C.); and Department of Chemistry, Qufu Normal University, Qufu 273165, P.R. China (H.L.)

Abstract

A simple flow injection chemiluminescence (CL) method for the determination of progesterone and hydrocortisones (HCRs) is described. The method is based on their sensitizing effect on the chemiluminescence reaction of a mercapto-group (-SH) with cerium(IV) in sulfuric acid. This method allows determination in the ranges 0.28–8.0 μg/mL for hydrocortisones (HCRs) and 0.5–8.0 μg/mL for progesterone with a detection limit of 0.10 μg/mL for HCRs and 0.26 μg/mL for progesterone. The experimental conditions for the reaction are optimized. The possible reaction mechanism is discussed.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

Link

http://journals.sagepub.com/doi/pdf/10.1366/0003702971939262

Reference11 articles.

1. High-Pressure Liquid Chromatographic Determination of Corticosteroids in Topical Pharmaceuticals

2. Determination of prednisolone, prednisone, and cortisol in human plasma by high-performance liquid chromatography

3. Fluorimetric Determination of Plasma Cortisol in Chronic Renal Failure

Cited by 5 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Chemiluminescent Emitter Based on the Reactions by Ce(IV);Analytical Letters;2008-11-18

2. Liquid-Phase Chemiluminescence Reactors;Journal of Analytical Chemistry;2004-02

3. Analytical applications of flow injection with chemiluminescence detection?a review;Luminescence;2001-01

4. Flow-chemiluminescence: a growing modality of pharmaceutical analysis;Luminescence;2001

5. Chemiluminescence determination of sodium 2-mercaptoethane sulfonate by flow injection analysis using cerium(IV) sensitized by quinine;Talanta;2000-05-05