Three-Dimensional Space- and Time-Resolved Fluorescence Spectroscopy

Author:

Sasaki Keiji1,Koshioka Masanori1,Masuhara Hiroshi1

Affiliation:

1. Microphotoconversion Project, ERATO, Research Development Corporation of Japan, Kyoto Research Park, Chudoji-minami, Shimogyo-ku, Kyoto 600, Japan

Abstract

A fluorescence spectroscopic system with both picosecond time resolution and submicrometer three-dimensional space resolution has been developed for elucidating the dynamics of inhomogeneous photophysical/photochemical phenomena. This system is based on a confocal laser scanning fluorescence microscope and a time-correlated single photon counting method. Results on demonstration experiments are shown to specify the performance of the system. Characteristics and limitations of the system are discussed.

Publisher

SAGE Publications

Subject

Spectroscopy,Instrumentation

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