Characterization of Metal Ion Binding Sites onDatura Innoxiaby Using Lanthanide Ion Probe Spectroscopy

Abstract

The excitation spectra associated with the7F0↦5D0transition of Eu+3has been used to examine the binding sites on cell wall fragments of Datura innoxia. Both native and esterified cell wall fragments were each examined at pH 5 and pH 2 to determine the contributions to metal ion sorption from both the carboxylate and sulfonate functional groups. The excitation spectra have been de-convoluted into the individual groups responsible for metal ion uptake. At least four unique binding sites can be described as being responsible for metal ion uptake. The higher affinity sites involve carboxylates in the binding of Eu+3in a tridentate (3:1 ligand-to-metal ratio) configuration.