Affiliation:
1. Siberian State Medical University, Tomsk
2. Institute of Cytology and Genetics SB RAS, Novosibirsk
Abstract
The aim of the study was to examine age-related changes in the ultrastructure of synapses in the CA1 region of the hippocampus of Wistar rats and premature aging OXYS rats.To assess changes of the sinaptoarhitectonics photographed on 15 randomly chosen fields of view of the pyramidal layer of the CA1 region of the hippocampus with five slices at a standard magnification of 10000. It was determined the amount of mineral contacts (size of the field of view is 50 μm2) and calculated numerical density of synapses per 100 μm2. The number of contacts with asymmetric and symmetric organization sistemasubsidiaries units, perforated, hypertrophic contacts are counted.The study showed that there are significant differences in the ultrastructural organization of the synapses between the lines, already 4 months old. The numerical density of snaps in this age group OXYS rats was significantly higher than that of Wistar. Ultrastructure of synapses in the CA1 region of the hippocampus of rats at the age of 18 months more bulupodadan pathological essentiaompetencies processes and membrane prematurely senescent rats in contrast to the control line. The numerical indicator of protect contents 18-month-old rats OXYS was significantly lower than in Wistar.Identified interstrain differences in the degree of changes of synapses can testify in favor of a different sensitivity to oxidative stress in rats investigated lines.
Publisher
Siberian State Medical University
Reference16 articles.
1. Nikolsky Ye.Ye. Molekuljarnye mehanizmy peredachi informacii cherez sinapsy himicheskogo tipa [Molecular mechanisms of information transmission through chemical type synapses]. Kazanskii medicinskii zhurnal – Kazan Medical Journal, 2010, vol. 91, no. 4, pp. 433–437.
2. Maksimova K.Yu., Stefanova N.A., Logvinov S.V. Morfologicheskie izmenenija v gippokampe krys pri prezhdevremennom starenii [Morphological changes in the hippocampus of rats in accelerated aging]. Byulleten' sibirskoy meditsiny – Bulletin of Siberian Medicine, 2014, vol. 13, no. 1, pp. 56–61.
3. Stefanova N.A., Muraleva N.A., Skulachev V.P., Koloso¬va N.G. Alzheimer's Disease-Like Pathology in Senescence-Accelerated OXYS Rats can be Partially Retarded with Mi-tochondria-Targeted Antioxidant SkQ1. Journal of Alzhei-mer's Disease, 2014, vol. 38, no. 3, pp. 681–694.
4. Masters C.L., Selkoe D.J. Biochemistry of amyloid β-protein and amyloid deposits in Alzheimer disease. Cold Spring Harbor Perspect Med., 2012, no. 2, pp. 62–69.
5. Adams J. Plasticity of the synaptic contact zone following loss ofsynapses in the cerebral cortex of aging humans. Brain Res., 1987, vol. 424, pp. 343–351.