Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860)

Author:

,Hui Jerome H. L.1ORCID,Chan Ting Fung2ORCID,Chan Leo Lai3ORCID,Cheung Siu Gin4ORCID,Cheang Chi Chiu56ORCID,Fang James Kar-Hei7ORCID,Gaitan-Espitia Juan Diego8ORCID,Lau Stanley Chun Kwan9ORCID,Sung Yik Hei1011ORCID,Wong Chris Kong Chu12ORCID,Yip Kevin Yuk-Lap1314ORCID,Wei Yingying15ORCID,So Wai Lok1ORCID,Nong Wenyan1ORCID,Pun Hydrogen Sui Fai16ORCID,Yau Wing Kwong16ORCID,Chiu Colleen Yuk Lin16ORCID,Chan Sammi Shan Shan16ORCID,Man Kacy Ka Ling16ORCID,Yip Ho Yin1ORCID

Affiliation:

1. School of Life Sciences, Simon F.S. Li Marine Science Laboratory, State Key Laboratory of Agrobiotechnology, Institute of Environment, Energy and Sustainability, The Chinese University of Hong Kong, Hong Kong, China

2. School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong SAR, China

3. State Key Laboratory of Marine Pollution and Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China

4. State Key Laboratory of Marine Pollution and Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China

5. Department of Science and Environmental Studies, The Education University of Hong Kong, Hong Kong SAR, China

6. EcoEdu PEI, Charlottetown, PE, C1A 4B7, Canada

7. Department of Food Science and Nutrition, Research Institute for Future Food, and State Key Laboratory of Marine Pollution, The Hong Kong Polytechnic University, Hong Kong SAR, China

8. The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China

9. Department of Ocean Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China

10. Science Unit, Lingnan University, Hong Kong SAR, China

11. School of Allied Health Sciences, University of Suffolk, Ipswich, IP4 1QJ, UK

12. Croucher Institute for Environmental Sciences, and Department of Biology, Hong Kong Baptist University, Hong Kong SAR, China

13. Department of Computer Science and Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China

14. Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA

15. Department of Statistics, The Chinese University of Hong Kong, Hong Kong SAR, China

16. Fung Yuen Butterfly Reserve, Hong Kong SAR, China

Abstract

The golden birdwing Troides aeacus (Lepidoptera, Papilionidae), a significant species in Asia, faces habitat loss due to urbanization and human activities, necessitating its protection. However, the lack of genomic resources hinders our understanding of their biology and diversity, and impedes our conservation efforts based on genetic information or markers. Here, we present the first chromosomal-level genome assembly of T. aeacus using PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (351 Mb) contains 98.94% of the sequences anchored to 30 pseudo-molecules. The genome assembly has high sequence continuity with contig length N50 = 11.67 Mb and L50 = 14, and scaffold length N50 = 12.2 Mb and L50 = 13. A total of 24,946 protein-coding genes were predicted, with high BUSCO score completeness (98.8% and 94.7% of genome and proteome BUSCO, respectively. This genome offers a significant resource for understanding the swallowtail butterfly biology and carrying out its conservation.

Funder

Hong Kong Research Grant Council Collaborative Research Fund

CUHK Strategic Seed Funding for Collaborative Research Scheme

CUHK Group Research Scheme

Publisher

GigaScience Press

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