Abstract
Background: The use of antioxidants of natural origin is an alternative to improve the cryopreservation of sheep semen. Objective: To evaluate the effect of Erythrina americana diet supplementation in the seminal characteristics of rams. Methods: Ten rams were randomly distributed in two treatments: l) group with addition of E. americana (20% dry matter basis) in the diet (AEA, n=5), and 2) the control group without addition of E. americana (SEA, n=5). Both diets were balanced iso-proteinically and iso-energetically. The feeding period of the rams was eleven weeks. The ejaculates were obtained through an artificial vagina and were evaluated fresh (37 °C) and refrigerated (5 °C). Volume (VOL) was evaluated with a tube graduated in milliliters, sperm concentration (CON x106) with a photometer and mass motility (MM, 1-5), individual motility (IM), normality (N) and live sperm (L) by microscopic observation. Results: The fresh semen showed that the inclusion of E. americana in the diet of rams did not modify (p>0.05) the parameters of VOL, CON, MM, and N, but decreased (p<0.05) the IM and L. In refrigerated semen the variables L and N were not different (p>0.05) among the treatment groups; however, IM was improved by the AEA group (p<0.05) compared to the SEA group. Conclusion: The supplementation with E. americana in the diet of rams reduces the sperm quality in fresh semen but is beneficial in the cooling process for refrigerated semen.
Reference40 articles.
1. Abadjieva D, Yotov S, Mladenova V, Lauberte L, Kalvanov I, Krasilnikova J, Telesheva G, Kistanova E. Positive effect of natural antioxidant oregonin from Alnus incana bark on ram semen quality stored at 5 °C for 48 h. Res Vet Sci 2020; 131:153-158. https://doi.org/10.1016/j.rvsc.2020.04.021
2. Abdi-Benemar H, Khalili B, Zamiri MJ, Ezazi H, Sotoudeh, AG, Hamidzadeh S, Simanoorf N. Effects of astaxanthin supplementation on the freezability, lipid peroxidation, antioxidant enzyme activities and post-thawing fertility of ram semen. Small Rum Res 2020; 192:106213. https://doi.org/10.1016/j.smallrumres.2020.106213
3. Allai L, Benmoula A, Maia MD, Nasser B, Amiri BE. Supplementation of ram semen extender to improve seminal quality and fertility rate. Anim Reprod Sci 2018; 192:6-17. https://doi.org/10.1016/j.anireprosci.2018.03.019
4. Al-Najar EM, Abdullah AM, Al-Rubaye TA, Hadi, SM. Evaluation of Marjoram Leaves (Oregano vulgar) as Feed Supplement on Quality of Semen in Awasian Pollination Rams. Arch Razi Inst 2022; 77(5):1831-1835. https://doi.org/10.22092/ari.2022.358366.2211
5. Association of Official Analytical Chemists (AOAC). Official methods of analysis Association Official Analytical Chemists. 20th Edition. Arlington, VA. 2017.