Biological properties of a newly isolated bacteriophage (NL1) that infects Escherichia coli O157:H7 strain

Abstract

Escherichia coli O157: H7 strain was used as the standard strain for bacteriophages isolated from polluted environmental foci and animal waste-contaminated waters, and some of the enteroinvasive Escherichia coli strains previously isolated from sporadic cases were used to determine the host diversity of phages. The physiological properties of bacteriophages specific to Escherichia coli O157:H7, designated Escherichiaphage NL1, were studied. After the isolation step, bacteriophages were purified and their titer was increased. Cross-antigenic properties of the hosts were investigated with Escherichia phage NL1 phage. One-step growth curve parameters such as adsorption time of phages, latent period, burst size, multiplicity of infection value (MOI) were determined. Results showed that bacteriophage NL1 completely lysed the E.coli O157:H7 (RSKK 09007) and E.coli O:164 RSKK 324. In the study, the effects of phage NL1 only on E.coli O157:H7 were investigated. The calculated highest titer was determined as Optimal multiplycity of infection (2.16x108). It was determined that the latent period lasted for 20 minutes and the phage was reached the burst size at approximately 90 minutes.