Identification of single nucleotide polymorphisms in GnRH-I, GnRH-II/MRPS26, GnRHR exons and their Association with egg production in Anand Synthetic White Leghorn and Anand Bantamised White Leghorn Chicken-Reference-Cited by-同舟云学术

Identification of single nucleotide polymorphisms in GnRH-I, GnRH-II/MRPS26, GnRHR exons and their Association with egg production in Anand Synthetic White Leghorn and Anand Bantamised White Leghorn Chicken

Published:2023-03-02 Issue:2 Volume:19 Page:94-98
ISSN:2395-1176
Container-title:Indian Journal of Veterinary Sciences & Biotechnology
language:
Short-container-title:ijvsbt

Author:

Pal Sourav^ORCID,Patel Ashish C.^ORCID,Hinsu Ankit T.^ORCID,Panchal Ketankumar J.^ORCID,Koringa Prakash G.^ORCID,Rank Dharamsibhai N.^ORCID

Abstract

The aim of study was to identify Single Nucleotide Polymorphisms (SNPs) in the exon regions of GnRH-I, GnRH-II/MRPS26 and GnRHR genes and to assess their association with egg production up to 64 weeks of age (EN64) in chicken. Blood samples were collected from 48 Anand Synthetic White Leghorn (ASWLH) and 48 Anand Bantamised White Leghorn (ABWLH) chicken. Custom panel was designed for these genes’ exon regions and amplicon sequencing was performed on Illumina Miseq platform. Total 41 SNPs (19 previously reported and 22 novel SNPs) were identified from 96 samples. Association analysis revealed that rs314996211 (p<0.05), rs313100310 (p<0.01), g.17308550T>C (p<0.01) of GnRHR were significantly associated with EN64. Conclusively, rs314996211, rs313100310, g.17308550T>C of GnRHR can be implemented as selection markers associated with EN64 in chicken.

Publisher

ACS Publisher

Subject

General Medicine

Reference13 articles.

1. AL-Jaryan, I. L. H., Walaa, S. H., & AL-Rekabi, M. M. (2021). Association of the melatonin receptor C gene with egg production traits in local Iraqi chicken. Systematic Reviews in Pharmacy, 12(1): 1406-1413.

2. Bhattacharya, T. K., Chatterjee, R. N., Dange, M., & Bhanja, S. K. (2019). Polymorphisms in GnRH I and GnRH II genes and their association with egg production and egg quality traits in chicken. British Poultry Science, 60(3): 187-194.

3. Durosaro, S. O., Ozoje, M. O., Adebambo, A. O., & Onagbesan, O. M. (2021). Identification and characterisation of single nucleotide polymorphisms in interferon regulatory factor-5 gene of Nigerian local chickens. Agricultura Tropica et Subtropica, 54(1): 1-13.

4. Fatemi, S. A., Mehrabani-Yeganeh, H., Nejati-Javaremi, A., & Niknafs, S. (2012). Association of neuropeptide Y and gonadotrophin-releasing hormone receptor gene SNPs with breeding value for growth and egg production traits in Mazandaran native chickens. Genetics and Molecular Research, 11(3): 2539-2547.

5. Gharaei, A., Rahdari, A., & Ghaffari, M. (2011). Induced spawning of Schizothoraxzarudnyi (Cyprinidae) by using synthetic hormones (Ovaprim and HCG). World Journal of Fish and Marine Sciences, 3(6): 518-522.