Study on the Discovery and Properties of Anticancer Fibrinolytic Active Protein from Sympiezomias velatus Chevrolat

Abstract

Sympiezomias velatus Chevrolat, commonly known as the cereal leaf beetle, is an insect pest that mainly affects tobacco crops. Feeding on tobacco leaves, it causes significant economic losses and impacts tobacco production. Due to its wide distribution and high adaptability, S. velatus has become an important model organism for studying croppest control and ecological adaptability. In this experiment, S. velatus was used as the research subject. The crude protein of S. velatus was isolated and extracted using the ammonium sulfate partial salting-out method. The protein activity was assessed using the fiber active plate method. The results demonstrated that three different concentration gradients of crude protein exhibited strong fibrinolytic activity. The optimum temperature for protein activity was found to be 35℃, while the optimal pH was approximately 7.0. Fe3+ significantly inhibited the activity of the fibrinolytic active protein in S. velatus, whereas Ba2+ showed a slight inhibitory effect on βME. Na+ and EDTA did not exhibit any impact. MTT experiments revealed that the fibrinolytic active protein displayed a certain inhibitory effect on cancer cell growth. This study provides valuable references for other research on S. velatus.