Author:
Popescu Sorina,Boldura Oana-Maria,Borozan Aurica,Madosa Emilian
Abstract
This paper aimed to evaluate 50 wheat samples collected from different western Romanian locations based on microbiological, molecular, and toxicogenic assays to determine their correlation when species of the genus Fusarium were analyzed. The presence of toxins determined by biochemical ELISA (Enzyme-Linked Immunosorbent Assay), the DNA analysis based on PCR (Polymerase Chain Reaction), and even accurate species identification using specific gene sequencing were used to evaluate the fungal early infection. Considering that in Romania the prevalence of Fusarium graminearum, and Fusarium proliferatum infections is the most important, it can be stated that the screening with primers specific to fungal species ensures a preliminary test for fungal infection identification before performing the test for mycotoxins.
Publisher
Centre for Evaluation in Education and Science (CEON/CEES)
Reference8 articles.
1. Alexander, N. J., McCormick, S. P., Waalwijk, C., Van der Lee, T., and Proctor, R. H. (2011). The genetic basis for 3-ADON and 15-ADON trichothecene chemotypes in Fusarium. Fungal Genetics and Biology. 48, 485-495;
2. Bozac, P., Popescu, S., Botau, D., Boldura, O.M., Pirvulescu, P. (2016) Molecular Characterization for some new Fusarium Isolates Collected from the West Part of Romania, Romanian Biotechnological Letters, 21(3), 11560-11568;
3. Geiser, D.M., Jimenez-Gasco, S., Kang, I., Makalowska, N., Veeraraghavan, T. J., Ward, N., Zhang, G.A., Kuldau, and O'Donnell K.. (2004). FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium. European Journal of Plant Pathology 110, 473-479;
4. Jurado M, Vazquez C, Patiño B, Gonzalez Jaen MT (2005). PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides. Systematic and Applied Microbiology, 28, 562-568;
5. Mesterhazy, A. (2002). Role of deoxynivalenol in aggressiveness of Fusarium graminearum and F. culmorum and in resistance to Fusarium head blight. Eur. J. Plant Pathol. 108, 675-684;
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献