Antifungal activity of aqueous and corn steep liquor extract of Ficus exasperata, Anonna muricata and Azadiractha indica-Reference-Cited by-同舟云学术

Antifungal activity of aqueous and corn steep liquor extract of Ficus exasperata, Anonna muricata and Azadiractha indica

Published:2020 Issue:40 Volume: Page:5-12
ISSN:0455-6224
Container-title:Lekovite sirovine
language:en
Short-container-title:Lekovite sirovine

Author:

Akinkugbe Temilola,Bankole Samuel,Ogunbamowo Paul,Awotedu Olamilekan

Abstract

This study investigated the activity of aqueous and corn steep liquor (CSL) extracts of Ficus exaperasta, Annona muricata and Azadirachta indica against Candida spp. isolated from high vaginal swab samples. Phytochemical screening of the plants was done using standard methods, the antifungal activity of the plant's extracts and standard drugs were tested against isolates of Candida spp. using the agar well diffusion method; the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were also determined using microdilution standardized techniques. Phytochemical screening of the aqueous and CSL extracts of the plants revealed the presence of tannin, saponin, flavonoids, and terpenoids. Among the five Candida strain isolates, the zone of inhibition produced by the plant extracts against C. albicans shows a range of 0-18.3 mm; C. krusei (strain A): 5.1-24.5 mm; C. krusei (strain B): 0-18.0 mm; C. kefyr (strain A): 6.1-27.5 mm; and C. kefyr (strain B): 0-22.0 mm. The CSL extract had higher inhibitory action compared with aqueous extract, also F. exasperata and A. muricata gave better antifungal activity against the tested Candida strains. The MIC of the aqueous and CSL extracts of the F. exasperata ranged between 6.25-12.50 mg/mL; A. muricata: 3.125-12.500 mg/mL, while the aqueous and CSL extracts of A. indica was found to have no activity at all the tested concentrations against C. albicans, C. krusei (strain A) and C. krusei (strain B), similar observation for the MFC. This study proved the antifungal efficacy of aqueous and CSL extracts of F. exasperata, A. muricata, and A. indica against isolates of Candida species which are usually implicated in candidiasis.

Publisher

Centre for Evaluation in Education and Science (CEON/CEES)

Subject

General Medicine

Link

https://scindeks-clanci.ceon.rs/data/pdf/0455-6224/2020/0455-62242040005A.pdf

Reference54 articles.

1. Abdus-Salaam, R. B., Adepoju, P. A., Olaleye, O. N. and Adeoye, I. A. (2014). Studies on the antimicrobial effect of corn steep liquor on some diarrhoea causing organisms, African Journal of Biotechnology 13(2): 332-335;

2. Adebayo, E., Ishola, O., Taiwo, O., Majolagbe, O. and Adekeye, B. (2009). Evaluations of the methanol extract of Ficus exasperata stem bark, leaf and root for phytochemical analysis and antimicrobial activities, African Journal of Plant Science 3(12): 283-287;

3. Ajayi, O. B., Oluyege, J. O., Olalemi, O. M. and Ilesanmi, T. M. (2012). Nutritional composition, phytochemical screening and antimicrobial properties of the leaf of Ficus exasperata (Vahl), Asian Journal of Biological and Life Sciences 1(3): 242-246;

4. Asticcioli, S., L., S., Daturi, R., Matti, C., Nucleo, E., Zara, F. and Pagani, L. (2009). Trends in frequency and in vitro antifungal susceptibility patterns of Candida isolates from women attending the STD outpatients clinic of a tertiary care hospital in Northern Italy during the years 2002-2007., The new Microbiologica 32(2): 199-204;

5. Bader, T., Bodendorfer, B., Schröppel, K. and Morschhäuser, J. (2003). Calcineurin is essential for virulence in Candida albicans, Infection and Immunity 71(9): 5344-5354;