Affinity of class I and class III peroxidases for H2O2 in pepper leaves of different maturity

Abstract

Paper deals with activities of Class III peroxidases (POX, EC 1.11.1.7) and Class I peroxidases (ascorbate peroxidase, APX, EC 1.11.1.11) as well as the Km of these enzymes for hydrogen peroxide (H2O2) in the pepper leaves of different maturity. The obtained results suggest that the youngest pepper leaves compared to more mature ones have different strategies for H2O2 removal. There was an increase in APX activity with leaf maturity, while POX activity had the opposite trend, and its lowest activity was observed in the oldest leaves. The accumulation of reducing substrates i.e., ascorbate and total phenols was in positive correlation with corresponding enzymes following leaf maturity. The comparison of POX and APX affinity for H2O2 in pepper leaves of different maturity and their relationship between these enzymes' activities were showed.