PCR diagnostics of pathogens of potato varieties (Solanum tuberosum)

Abstract

When creating technological schemes for the improvement of potatoes, PCR methods are currently actively used, in particular, amplification with hybridization-fluorescence detection in the "real time" and "end point" modes. In this work, both of these methods were applied and the diagnostics of cultivated potato varieties and lines for the presence of viral pathogens A, M, S, X, Y, Andean mottle viruses and latent potato thymovirus (APMV, APLV), as well as tuber spindle viroid (PSTVd) and potato panicle top virus (PMTV) was carried out. The analysis for the presence of parasitic diseases of pale Globodera pallida and golden cyst nematodes Globodera rostochiensis, bacterial ring and brown rot Clavibacter michiganensis subsp. sepedonicus and Ralstonia solanacearum, as well as fungal cancer Synchytrium endobioticum. As a result of PCR analysis, parasitic, fungal and bacterial diseases were not detected 44% of the total number of samples were healthy from viral diseases, while 25% were initially infected and underwent sanitation operations. For the first time, the PCR diagnostic system was introduced into the technological processes for obtaining varietal crops of the Tyumen region, free from diseases, to adapt to growing conditions in Western Siberia.