Effect of Kaempferol, Diminazene Aceturate and their Combination on Hematological Parameters in Trypanosoma

Abstract

Kaempferol is a polyphenolic compound and are widely distributed in plants. It is used in the treatment of different disease conditions. With the endemic resistant parasites against most antitrypanosomal agents and the toxicity associated with diminazene aceturate, the search for safer and more effective alternative therapy of trypanosomosis becomes paramount. In this study the effect of treatment with kaempferol and diminazene aceturate on Hematological parameters in mice with experimental Trypanosoma brucei brucei infection was evaluated. Thirty six adult swiss albino mice of either sex were randomly divided into six groups of six mice each. Mice in group I were untreated uninfected. Mice in group II were pre-treated with kaempferol (1 mg/kg) for 14 days. Mice in groups II to VI each were inoculated with blood containing Trypanosoma brucei brucei (106 trypanosomes/ml of blood/animal) intraperitoneally. Following establishment of the infection (four days post-inoculation), mice in group III were treated once with diminazene aceturate (3.5 mg/kg) I.P. Mice in group IV were treated with diminazene aceturate (3.5 mg/kg) once I.P, and then continued with kaempferol (1 mg/kg) for nine days.