Assessment of the photosensitizer efficacy after determination of threshold doses initiating erythrocyte hemolysis

Author:

Alekseev Yu. V.1ORCID,Mislavsky O. V2ORCID,Duvanskiy V. A.3ORCID,Duvanskiy R. A.4ORCID,Drozdova N. V.1ORCID

Affiliation:

1. Skobelkin State Scientific Center of Laser Medicine

2. Skobelkin State Scientific Center of Laser Medicine; National Research Center – Institute of Immunology

3. Skobelkin State Scientific Center of Laser Medicine; Peoples’ Friendship University of Russia

4. Skobelkin State Scientific Center of Laser Medicine; Treatment and Rehabilitation Center of National Medical Research Center

Abstract

Purpose: To find out a technique for determining the effectiveness of porphyrin-type photosensitizers with concentrations which cause hemolysis in erythrocytes (hemolysis threshold). The hemolysis threshold is found under the following conditions: irradiation in the Soret band with standard parameters – power density, exposure dose, irradiation time.Material and methods. Phototherapeutic device “AST” (LLC “Pankov-medical”) – average power 0.5 W, wavelength ≈ 405 nm. Rat blood erythrocytes. “Multiscan MS” device manufactured by Labsystems, Finland. Preparation “Dimegin”, hematoporphyrin derivative; preparation “Photoditazine” (LLC “VETA-GRAND”), chlorine E6 derivative.Results. On analyzing the results obtained after experimentation with the abovementioned two preparations, it was shown that the developed technique has a high efficacy. It was also shown that the studied photosensitizers are equally effective when irradiated in the Soret band.Conclusion. The developed technique is simple and easy to use. It helps to assess the efficacy of various photosensitizers thus, facilitating the selection of their necessary concentrations for managing different diseases.

Publisher

Skobelkin State Scientific Center of Laser Medicine

Subject

General Medicine

Reference24 articles.

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3. Yakubovskaya R.I., Plyutinskaya A.D., Lukyanets E.A. Comparative study of photosensitizers of different classes in an in vitro system. Phthalocyanines. Rossiyskiy bioterapevticheskiy zhurnal. 2014. 13 (4): 65–72. [In Russ.].

4. Makarova E.A., Yakubovskaya R.I., Vorozhtsov G.N., Lastovoy A.P., Lukyanets E.A., Morozova N.B., Plotnikova E.A. Photosensitizer for photodynamic therapy. Patent for invention RU 2549953 C2, 05.10.2015. Application N 2013157611/15 dated 25.12.2013. [In Russ.].

5. Bondarenko V.M., Alekseev Yu.V., Mislavsky O.V., Ponomarev G.V. Prospects for the application of 2,4-di(1- methoxyethyl)-deuteroporphyrin-IX (“dimegin”) disodium salt for photodynamic therapy of non-oncological diseases. Biomeditsinskaya khimiya. 2014; 60 (3): 338–347. [In Russ.].

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