Affiliation:
1. National Medical Research Center of Cardiology of the Ministry of Health
Abstract
Aim. To assess the influence of coronary heart disease patients (CHD) therapy with statins or PCSK9 inhibitors influence on the level of oxidatively modified low density lipoproteides (LDL) and activity of erythrocyte Se-glutathione peroxidase (GSH-Px).Material and methods. To the study, CHD patients were included (9-10 males per group), who during 6 months were undergoing statin therapy — 40 mg per day of pravastatin (group 1) or 0,4 mg per day of cerivastatin (group 2), as the therapy with PCSK9 inhibitor — 420 mg per month evolocumab (group 3) during 1 year. The level of lipohydroperoxide in LDL (LOOH-LDL) was measured in the groups 1 and 2 with the modified method and usage of Fe-xilenolorange; content of oxidized LDL (oxLDL) in the group 3 — with immune chemistry method (assays Mercodia, Sweden). Activeness of GSH-Px in all groups was assessed with the modified methods bound with glutathione reductase system and tret-buthyl hydroperoxide as a substrate.Results. Simultaneously with the decrease of LDL cholesterol in the groups 1 and 2 there was significant increase (in the group 2 — 6-7 times in 3-6 months of therapy) of the level of LOOH-LDL. In the group 2 there was marked significant decrease of GSH-Px activity beginning from the month 3. In the group 3, with decreased LDL cholesterol there was significant decline in oxLDL with changed activity of GSH-Px.Conclusion. Statins, effectively decreasing the level of LDL cholesterol, simultaneously induce the oxidation of LDL and decrease the activity of GSH-Px. Inhibitor PCSK9 not only does effectively decrease the level of LDL cholesterol, but also the content of oxLDL, not leading to decreased GSH-Px activity.
Subject
Cardiology and Cardiovascular Medicine
Reference14 articles.
1. Menshchikova EB, Zenkov NK, Lankin VZ, et al. Oxydative stress. Pathological states and diseases. Novosibirsk. ARTA. 2008. 284p. (In Rus)
2. Lankin VZ, Tikhaze AK, Konovalova GG, et al. Aldehyde-dependent modification of lowdensity lipoproteins. In: Handbook of Lipoprotein Research, N.Y., Nova Sci. 2010:85-107. ISBN10:1616681861.
3. Lankin VZ, Tikhaze AK. Role of Oxidative Stress in the Genesis of Atherosclerosis and Diabetes Mellitus: A Personal Look Back on 50 Years of Research. Сurr Аging Sci. 2017;10(1):18-25. doi:10.2174/1874609809666160926142640.
4. Pedersen TR. Pleiotropic effects of statins: evidence against benefits beyond LDL-cholesterol lowering. Am J Cardiovasc Drugs. 2010;10(Suppl1):10-17. doi:10.2165/1158822-S0-000000000-00000.
5. Lankin VZ, Ivanova MV, Konovalova GG, et al. Effect of beta-hydroxy-beta-methylglutaryl coenzyme A reductase inhibitors and antioxidant vitamins on free radical lipid oxidation in rat liver. Bull Exp Biol Med. 2007;143:414-7. (In Russ.)
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