Comparative study of methods for the modernization of mesh prostheses as a matrix for the cultivation of fibroblasts

Abstract

Objective: conduct a comparative study of methods for the modernization of mesh prostheses as a matrix for the cultivation of fibroblasts. Materials and methods: In the experiment conditions, polycaprolactone substance was applied to the surface of mesh hernioendoprostheses according to the original method proposed by V.V. Bereshchenko and a team of co-authors (2019). The polyvinylidene fluoride "Uniflex Standard" prosthesis (Lintex LLC, St. Petersburg, Russia) was chosen as the object of study. After applying the polymer, low-temperature plasma treatment was performed with the characteristics: frequency 5 kHz, pulse voltage 10 kV, power density 2 W/cm2, exposure time 5 minutes. After that, the samples were sterilized using X-ray radiation with a power of 150 kV and an exposure of 3 min. The application of cells was carried out by introducing samples into a DMEM medium with fibroblast culture for 5 days. The obtained samples were studied using scanning electron microscopy. Statistical processing was performed using the nonparametric Mann-Whitney test in the Statistica 10 program (Dell Software Company, Round Rock, Texas, United States of America). Results: The data obtained indicate the successful attachment of fibroblasts to the surface of modified hernioprostheses.