Inconsequential role for chemerin‐like receptor 1 in the manifestation of ozone‐induced lung pathophysiology in male mice

Author:

Johnston Richard A.1234ORCID,Pilkington Albert W.1,Atkins Constance L.5,Boots Theresa E.1,Brown Philip L.1,Jackson William T.3,Spencer Chantal Y.6,Siddiqui Saad R.3,Haque Ikram U.37

Affiliation:

1. Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention United States Department of Health and Human Services Morgantown West Virginia USA

2. Section of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, School of Medicine West Virginia University Morgantown West Virginia USA

3. Division of Critical Care Medicine, Department of Pediatrics McGovern Medical School at the University of Texas Health Science Center at Houston Houston Texas USA

4. Department of Integrative Biology and Pharmacology McGovern Medical School at the University of Texas Health Science Center at Houston Houston Texas USA

5. Division of Pulmonary Medicine, Department of Pediatrics McGovern Medical School at the University of Texas Health Science Center at Houston Houston Texas USA

6. Section of Pediatric Pulmonology, Department of Pediatrics Baylor College of Medicine Houston Texas USA

7. Division of Critical Care, Department of Pediatrics Sidra Medicine Doha Qatar

Abstract

AbstractWe executed this study to determine if chemerin‐like receptor 1 (CMKLR1), a Gi/o protein‐coupled receptor expressed by leukocytes and non‐leukocytes, contributes to the development of phenotypic features of non‐atopic asthma, including airway hyperresponsiveness (AHR) to acetyl‐β‐methylcholine chloride, lung hyperpermeability, airway epithelial cell desquamation, and lung inflammation. Accordingly, we quantified sequelae of non‐atopic asthma in wild‐type mice and mice incapable of expressing CMKLR1 (CMKLR1‐deficient mice) following cessation of acute inhalation exposure to either filtered room air (air) or ozone (O3), a criteria pollutant and non‐atopic asthma stimulus. Following exposure to air, lung elastic recoil and airway responsiveness were greater while the quantity of adiponectin, a multi‐functional adipocytokine, in bronchoalveolar lavage (BAL) fluid was lower in CMKLR1‐deficient as compared to wild‐type mice. Regardless of genotype, exposure to O3 caused AHR, lung hyperpermeability, airway epithelial cell desquamation, and lung inflammation. Nevertheless, except for minimal genotype‐related effects on lung hyperpermeability and BAL adiponectin, we observed no other genotype‐related differences following O3 exposure. In summary, we demonstrate that CMKLR1 limits the severity of innate airway responsiveness and lung elastic recoil but has a nominal effect on lung pathophysiology induced by acute exposure to O3.

Funder

National Institute of Environmental Health Sciences

Division of Intramural Research

Publisher

Wiley

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