Revisiting pancreatic islet isolation in murine models: A practical and effective technical protocol

Author:

Fernandes‐da‐Silva Aline1ORCID,Miranda Rosiane Aparecida2ORCID,Lisboa Patricia Cristina2ORCID,Souza‐Mello Vanessa1ORCID

Affiliation:

1. Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology Roberto Alcantara Gomes State University of Rio de Janeiro Rio de Janeiro Brazil

2. Laboratory of Endocrine Physiology, Department of Physiological Sciences, Institute of Biology Roberto Alcantara Gomes State University of Rio de Janeiro Rio de Janeiro Brazil

Abstract

AbstractThe endocrine pancreas is composed of clusters of cell groups called pancreatic islets. These cells are responsible for the synthesis and secretion of hormones crucial for glycemic homeostasis, such as insulin and glucagon. Therefore, these cells were the targets of many studies. One method to study and/or understand endocrine pancreatic physiology is the isolation of these islets and stimulation of hormone production using different concentrations of glucose, agonists, and/or antagonists of specific secretagogues and mimicking the stimulation of hormonal synthesis and secretion. Many researchers studied pancreatic physiology in murine models due to their ease of maintenance and rapid development. However, the isolation of pancreatic islets involves meticulous processes that may vary between rodent species. The present study describes a simple and effective technical protocol for isolating intact islets from mice and rats for use as a practical guide for researchers. The method involves digestion of the acinar parenchyma by intraductal collagenase. Isolated islets are suitable for in vitro endocrine secretion analyses, microscopy techniques, and biochemical analyses.

Funder

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Wiley

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