Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro-Reference-Cited by-同舟云学术

Harmaline induces apoptosis and inhibits migration in A2780 ovarian cancer cells in vitro

Published:2024-03 Issue:6 Volume:12 Page:
ISSN:2051-817X
Container-title:Physiological Reports
language:en
Short-container-title:Physiological Reports

Author:

Shariat Razavi Seyed Ali¹,Taghdisi Khaboushan Masoumeh²,Jafari Raha³,Shahini Arshia⁴,Ferns Gordon A.⁵,Bahrami Afsane⁶⁷^ORCID

Affiliation:

1. Department of Neuroscience, Faculty of Medicine Mashhad University of Medical Sciences Mashhad Iran

2. Student Research Committee Islamic Azad University Mashhad Branch Mashhad Iran

3. Department of Medicine, Mashhad Medical Sciences Branch Islamic Azad University Mashhad Iran

4. Department of Laboratory Sciences, School of Allied Medical Sciences Arak University of Medical Sciences Arak Iran

5. Division of Medical Education Brighton and Sussex Medical School Falmer, Brighton, Sussex UK

6. Clinical Research Development Unit, Imam Reza Hospital, Faculty of Medicine Mashhad University of Medical Sciences Mashhad Iran

7. Clinical Research Development Unit of Akbar Hospital, Faculty of Medicine Mashhad University of Medical Sciences Mashhad Iran

Abstract

AbstractOvarian cancer is one of the most prevalent malignancies in women. Harmaline is reported to have powerful anticancer properties. We aimed to investigate the apoptotic and antimetastatic properties of harmaline in A2780 ovarian cancer cells. Cell viability, apoptosis, migration, and invasion were investigated in cells treated with harmaline. Reactive oxygen species (ROS) production, mRNA expression of apoptosis‐associated genes, MMP‐2, and MMP‐9 were measured. Harmaline attenuated the viability of A2780 ovarian cancer cells in a dose‐ and time‐dependent way. Furthermore, compared to NIH/3T3 mouse normal cell line (IC50 = 417 μM), the malignant A2080 cells were more sensitive to harmaline (IC50 = 300 μM after 24 h). Harmaline increased the production of ROS, raised the mRNA expression of p53 and the Bax/Bcl2 ratio. Harmaline also increased the proportion of cells in the late apoptotic and necrotic phases. MMP‐2 and MMP‐9's mRNA expression, gelatinase activity, and migration of A2780 cells also decreased by harmaline. These findings suggest that harmaline may have the potential to be a therapeutic drug for ovarian cancer by triggering apoptosis and suppressing invasion and migration.

Funder

Mashhad University of Medical Sciences

Publisher

Wiley

Link

https://physoc.onlinelibrary.wiley.com/doi/pdf/10.14814/phy2.15984

Reference34 articles.

1. MT1-MMP and MMP-2 mRNA expression in human ovarian tumors: Possible implications for the role of desmoplastic fibroblasts

2. Chemistry, pharmacology and medicinal properties of Peganum harmala L;Asgarpanah J.;African Journal of Pharmacy and Pharmacology,2012

3. Therapeutic potential of harmaline, a novel alkaloid, against cervical cancer cells in vitro: Apoptotic induction and DNA interaction study;Bhattacharjee P.;Journal of Applied Biology and Biotechnology,2018

4. Genotoxic Effects of the Alkaloids Harman and Harmine Assessed by Comet Assay and Chromosome Aberration Test in Mammalian Cells in vitro