ISOLATION AND STUDY OF THE POLYSACCHARIDE COMPLEX ISOLATED FROM AESCULUS HIPPOCASTA-NUM L.

Abstract

This paper presents the results of a study of the polysaccharide complex, first obtained using microwave radiation from the shells of horse chestnut seeds (Aesculus Hippocastanum L.), collected on the territory of the Tashkent region of the Republic of Uzbekistan. It is shown that the use of microwave waves makes it possible to intensify the method of obtaining polysaccharides with an increase in their yield. The yield of the obtained product reaches 13%, compared with the method of obtaining without using microwave radiation (9.85%), which indicates the prospects of using microwave radiation in the isolation of polysaccharides from this type of raw material. The molecular weight characteristics were determined, and using GC/MS analysis of trimethylsilyl derivatives, the monosaccharide composition of the complex isolated from the shells of the Semyon chestnut by alkaline extraction was established. It was found that the isolated polysaccharides consist mainly of arabinose and galactose residues (65–100%), and also, as minor monosaccharides, up to 35% of glucose, xylose, mannose, rhamnose, ribose residues. Signals and absorption bands corresponding to total polysaccharides were observed in 13C NMR and IR spectra. The NMR analysis data are consistent with the results of the analysis of the monosaccharide composition of the polysaccharide complex obtained using GC / MS. The antiradical activity of the polysaccharide complex has been established.