Author:
Gavrilov Anatoliy Broneslavovich,Goryainov Sergey Vladimirovich,Marinichev Anton Alekseyevich,Gessler Natal'ya Nikolayevna,Klyayn Ol'ga Ivanovna,Isakova Yelena Pavlovna,Deryabina Yuliya Ivanovna
Abstract
A total polyphenol content and total antioxidant activity in the extracts isolated from coniferous wood and bark, namely common spruce (Picea abies), common pine (Pinus sylvestris), cedar pine (Pinus sibirica), siberian larch (Larix sibirica), juniper (Juniperus communis) from 7 regions of the European Russia were under study. Extraction of polyphenols was performed using a 20% ethyl alcoholic solution with a VER-200 extractor. The total polyphenol content was performed spectrophotometrically using the Folin-Ciocalteu reagent. The total antioxidants capacity was assayed with a Tsvet-Yauza-01-AA liquid chromatograph to assess the extract oxidation on the working electrode surface. Gallic acid served as the standard. For polyphenolic components identification we used the gas chromatography-mass spectroscopy with 42 standard samples of phenolic and polyphenolic compounds as the standards. 15 compounds of phenolic nature, namely salicylic and ferulic acids, stilbenes of resveratrol and isoranaptigenin, flavonoids of catechin, catechol, dihydroquercetin, quercetin, dihydrokempferol, kempferol, dihydromyrcetin, luteolin, apigenin, chrysin, pinocembrin. The extracts from common spruce (Picea abies), common pine (Pinus sylvestris), and cedar pine (Pinus sibirica) from the Eastern Russian regions, namely Perm and Vologda regions. The application of Russian conifers bark and wood is concluded to be a promising source of biologically active polyphenols.
Subject
Organic Chemistry,Plant Science,Biomaterials
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