ANTIRADICAL ACTIVITY OF SEPARATE FRACTIONS OF ESSENTIAL OIL OF ACORUS CALAMUS ROOTS

Abstract

Essential oil from the roots of calamus marsh (Acorus calamus L) was isolated by the method of exhaustive hydroparodistillation, growing on the Krasnoyarsk territory. Separate fractions of essential oil were obtained depending on the duration of isolation. The component composition of the essential oil, the main components of which are acorenone, butyrate α-terpineol, germacrene B, camphor. The antiradical properties of the essential oil of A. сalamus roots growing in the Siberian region, as well as the antiradical properties of its individual fractions were studied for the first time in order to determine the most promising samples as sources of biologically active complexes. To determine the antiradical activity, the reaction of the essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. The antiradical activity of all studied samples of essential oils was established. There was an increase in antiradical activity with a decrease in the content of monoterpenes in the composition of the essential oil. The lowest antiradical activity (17.5%) was shown by the fraction of essential oil with a high content of monoterpenes, the highest (~100%) – fractions with a high content of oxygen-containing compounds. It was revealed that the ability of components of individual fractions of A. сalamus essential oil to inhibit DPPH radicals is comparable with the antiradical activity of ascorbic acid solutions of equivalent concentration.