COMPOSITION AND BIOLOGICAL ACTIVITY OF FLAVONOIDS ANF HYDROXYCINNAMATES OF URTICA CANNABINA (URTICACEAE)

Abstract

Phenolic compounds of Urtica cannabina L. (cannabis nettle; family Urticaceae), a widespread Asian species of Russia, are poorly studied. In the present study, for the first time, a chromatographic analysis of U. cannabina growing in Eastern Siberia was realized together with isolation of flavonoids and hydroxycinnamates and study of the biological activity of extracts and pure compounds. As a result, it was found that the total extracts of U. cannabina were characterized by a high content of phenolic compounds and, as a result, a pronounced antiradical activity against DPPH• and ABTS•+ radicals. After chromatographic separation, the presence of 24 compounds was detected in the plant, of which 22 were identified for the first time for the species, including a new flavonoid, which was kaempferol-3-O-(2'',6''-di-O-acetyl)-β-D-glucopyranoside (astragalin 2'',6''-di-O-acetate) based on UV, NMR spectroscopy and mass spectrometry data. Flavonoids of U. cannabina showed a pronounced antioxidant effect on the model of lipopolysaccharide-induced nitric oxide production in macrophages. Studies have shown that U. cannabina is a source of biologically active phenolic compounds.