INVESTIGATION OF CELANDINE EXTRACTS (CHELIDONIUM MAJUS L.) BY 1H NMR AND QNMR METHODS

Author:

Korotkikh Mikhail Olegovich,Romanenko Elena Petrovna,Tikhova Vera Dmitriyevna,Tkachev Aleksej Vasil'evichORCID

Abstract

The analysis of Celandine extracts (Chelidonium majus L.) by 1H NMR method makes it possible to identify the main alkaloids – derivatives of phenanthridine, protoberberin and protopin, and the use of the qNMR method provides information with satisfactory accuracy about the content of these alkaloids. The content of alkaloids determined in the composition of Celandine extracts by the qNMR method (0.35%) is 2 times lower than when using the spectrophotometric determination procedure described in the GF of the Russian Federation XIV (0.72%). The extraction results according to the methods given in the State Pharmacopoeia of the Russian Federation of the XIV edition and in the State Pharmacopoeia of the USSR of the IX edition give similar results: the same alkaloids are found in the extracts – chelidonin, stylopine and protopine, and the total content of alkaloids is 1.6-1.8 times less than when extracting the same raw material samples by the method insistence. When extracted by infusion, the main alkaloids in the extracts are dihydrosanguinarin, chelidonin, stylopine, berberine and coptisin.

Publisher

Altai State University

Subject

Organic Chemistry,Plant Science,Biomaterials

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