IN VITRO EVALUATION OF BOSWELLIA SPP. ANTI-BIOFILM ACTIVITY

Abstract

Recent years have seen an increase in microbial biofilm resistance, which has led to great challenges for the medical community in terms of treating diseases and the food industry in terms of contamination and the loss of shelf life. The purpose of this work is to test the antimicrobial efficacy against the biofilm formed by the following reference strains: Streptococcus pyogenes (ATCC 19615), Staphylococcus aureus (ATCC 25923), Shigella flexneri (ATCC 12022), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli ( ATCC 25922), Salmonella typhimurium (ATCC 14028), Haemophilus influenzae type B (ATCC 10211), Listeria monocytogenes (ATCC 19114), Bacillus cereus (ATCC 10876) Clostridium perfringens (ATCC 13124), Candida parapsilopsis (ATCC 22019) and Candida albicans ( ATCC 10231) as well as the MBIC evaluation of the essential oil of Boswellia spp. The evaluation was performed by evaluating the loss of microbial mass by measuring the OD by spectrophotometry in accordance with ISO 20776- 1:2019. The statistically evaluated results suggest a very good efficacy against Grampositive bacteria and fungi and a more limited activity against Gram-negative bacteria.