SELECTION OF CONDITIONS FOR CULTIVATION OF MICROALGAE- CARBON SEQESTRATORS

Abstract

The use of microalgae as carbon sequestrators requires optimization of the cultivation process. Growth curves for three types of microalgae were plotted. The growth was carried out at different temperatures 30 and 35 ? and with initially different concentrations of inoculum 30 and 3% in order to assess the possibility of growing crops at the lowest cost and with a high yield of a useful product. We used 3 strains of microalgae - potential sequestrators. The introduction of the inoculum at a dose of 30% led to an acceleration of the growth curve reaching a plateau on day 5 at an incubation temperature of 30 ?. At a dose of 3% inoculum, growth is uniform for all species at 30 degree Celsius, while at 30% inoculation, Strain 2 shows a decrease in optical density and growth retardation. It is likely that the reasons for growth restriction are either due to a lack of nutrients for a given species, which leads to intraspecific competition. It was found that the temperature of 35 ? for Strain 3 was optimal, the growth curve quickly reached a plateau and reached a higher optical density. Increasing the dose of inoculum at 35 ? did not lead to a significant increase in optical density. Thus, from an economic point of view, it is optimal to grow microalgae at 35 ? and an inoculum dose of 3%.