An Infected Wound Simulation in Rats with Long-Term Diabetes Mellitus

Abstract

Introduction. The prevalence of infected wounds of the lower extremities combined with type II diabetes mellitus is 4-5 patients per 1000 population in industrialised countries. There is a steady trend towards an increase in this group of patients. It is necessary to study and evaluate the effectiveness of new treatment methods of purulent-necrotic complications in patients with diabetes mellitus complicated by diabetic foot syndrome (DFS). The aim of study was to simulate a long-term diabetes mellitus and an infected wound.Materials and methods. The article presents a technique for long-term diabetes mellitus (DM) and an infected wound simulation in long-term diabetes mellitus (DM) in Wistar rats. Long-term DM was simulated based on the technique described in the author's patent of the Russian Federation No. 2735708, 2020. Diabetes mellitus was simulated in 120 animals. The infected wound was simulated in 30 Wistar rats with alloxon-remaxol-induced long-term DM and in 5 healthy animals. Results. Blood sugar in animals with diabetes mellitus was significantly higher compared to animals in the control group. A consistently high level of sugars throughout the experiment evidenced presence of DM in animals of the second and third groups. In 7 days of the experiment, an infected wound with histological signs of an infected wound was formed in the rats.Conclusion. The effectiveness of remaxol application as a hepatoprotector and antioxidant in of alloxone DM simulation has been evidenced; this allows simulating the disorder with consistently high sugars and prolong life of experimental animals for the period necessary to fulfill scientific tasks set in the experiment.